- ￥2210
- biorbyt
- orb1499369
- 英国
- 2025年12月08日
- FC, ICC, WB
- Rabbit
- Human
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- 详细信息
- 技术资料
- 抗体名：
GATA1 Recombinant Rabbit Monoclonal Antibody抗体
- 抗体英文名：
GATA1 Recombinant Rabbit Monoclonal Antibody
- 靶点：
GATA1
- 浓度：
1mg/ml
- 应用范围：
FC, ICC, WB
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1499369
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Recombinant
- 保存条件：
参考说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
Recombinant protein within human GATA1 (1-250/413aa)
- 规格：
25 ul
产品描述：GATA1 Recombinant Rabbit Monoclonal Antibody
别名:GATA1; ERYF 1; ERYF1 antibody Erythroid transcription factor; Erythrold transcription factor 1; GATA 1; GATA binding factor 1; GATA binding protein 1; GF 1; GF1; Globin transcription factor 1; NF E1; NF E1 DNA binding protein; NFE 1; NFE1; GATA1_HUMAN; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein.
免疫原：Recombinant protein within human GATA1 (1-250/413aa)
预测反应性:Human
克隆性:6B12
分子量:45 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=1:50-100, ICC/IF=1:50-200
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P15976
Note:For research use only.
别名:GATA1; ERYF 1; ERYF1 antibody Erythroid transcription factor; Erythrold transcription factor 1; GATA 1; GATA binding factor 1; GATA binding protein 1; GF 1; GF1; Globin transcription factor 1; NF E1; NF E1 DNA binding protein; NFE 1; NFE1; GATA1_HUMAN; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein.
免疫原：Recombinant protein within human GATA1 (1-250/413aa)
预测反应性:Human
克隆性:6B12
分子量:45 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=1:50-100, ICC/IF=1:50-200
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P15976
Note:For research use only.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-GATA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499369, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GATA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499369, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GATA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499369, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of GATA1 on K562 cell lysates with Rabbit anti-GATA1 antibody (orb1499369) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa, Observed band size: 50 kDa, Exposure time: 30 seconds, 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (orb1499369) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300000 dilution was used for 1 hour at room temperature.
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GATA1 Recombinant Rabbit Monoclonal Antibody抗体，orb1499369，biorbyt
￥2210
