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NCOA4 (IN) Antibody抗体,orb12380

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  • ¥3770
  • biorbyt已认证
  • orb1238076
  • 英国
  • 2025年12月08日
  • ELISA, IF, IHC, WB
  • Human, Mouse, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      NCOA4 (IN) Antibody抗体

    • 抗体英文名

      NCOA4 (IN) Antibody

    • 靶点

      NCOA4

    • 浓度

      1 mg/mL

    • 应用范围

      ELISA, IF, IHC, WB

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb1238076

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      Anti-NCOA4 antibody (orb1238076) was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.

    • 规格

      0.02 mg

    产品描述:NCOA4 (IN) Antibody

    别名:NCOA4 Antibody: Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, Androgen receptor-associated protein of 70 kDa, Ret-activating protein ELE1, ARA70, ELE1, RFG

    免疫原:Anti-NCOA4 antibody (orb1238076) was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The 免疫原 is located within 420-470 amino acids of NCOA4.

    预测反应性:Canine, Equine, Monkey, Primate

    分子量:Predicted: 70-74 kDObserved: 70-74 kD

    防腐剂:NCOA4 Antibody is supplied in PBS containing 0.02% sodium azide.

    纯化:NCOA4 Antibody is affinity chromatography purified via peptide column.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    NCBI:Q13772

    UniProt ID:Q13772

    Note:For research use only.

    产品细节图片1
    Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: NCOA4 orb1238076, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

    产品细节图片2
    Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: NCOA4 orb1238076, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

    产品细节图片3
    Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: NCOA4 orb1238076, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

    产品细节图片4
    Immunofluorescence Validation of NCOA4 in Daudi Cells. Immunofluorescent analysis of 4% Fixative-fixed Daudi cells labeling NCOA4 with orb1238076 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

    产品细节图片5
    Immunohistochemistry Validation of NCOA4 in Human lung. Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (orb1238076) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    产品细节图片6
    Immunohistochemistry Validation of NCOA4 in Mouse lung. Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (orb1238076) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    产品细节图片7
    Immunohistochemistry Validation of NCOA4 in Rat lung. Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (orb1238076) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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