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- 详细信息
- 技术资料
- 抗体名:
EBI3 Antibody抗体
- 抗体英文名:
EBI3 Antibody
- 靶点:
EBI3
- 浓度:
1 mg/mL
- 应用范围:
ELISA, IF, IHC-P, WB
- 适应物种:
Human
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb1238976
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
Anti-EBI-3 antibody (orb1238976) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3. The immunogen is located within the last 50 amino acids of EBI3.
- 规格:
0.02 mg
产品描述:EBI3 Antibody
别名:EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta
免疫原:Anti-EBI-3 antibody (orb1238976) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3. The 免疫原 is located within the last 50 amino acids of EBI3.
预测反应性:Mouse, Rat
分子量:Predicted: 25kDObserved: 30 kD (25kD + 2 N-Glycosylations)
防腐剂:EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.
纯化:EBI3 Antibody is affinity chromatography purified via peptide column.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:NP_005746
UniProt ID:Q14213
Note:For research use only.



别名:EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta
免疫原:Anti-EBI-3 antibody (orb1238976) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3. The 免疫原 is located within the last 50 amino acids of EBI3.
预测反应性:Mouse, Rat
分子量:Predicted: 25kDObserved: 30 kD (25kD + 2 N-Glycosylations)
防腐剂:EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.
纯化:EBI3 Antibody is affinity chromatography purified via peptide column.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:NP_005746
UniProt ID:Q14213
Note:For research use only.

Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane. Antibodies: EBI-3 orb1238976, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL, Lane 2: 2 µg/mL, Lane 3: 4 µg/mL.

Immunohistochemistry Validation of EBI-3 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded Human Spleen tissue using anti-EBI-3 antibody (orb1238976) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of EBI-3 in Human Spleen Tissue. Immunofluorescent analysis of 4% Fixative-fixed Human Spleen tissue labeling EBI-3 with orb1238976 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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EBI3 Antibody抗体,orb1238976,biorbyt
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