- ￥3770
- biorbyt
- orb1239042
- 英国
- 2025年12月08日
- ELISA, IF, IHC-P, WB
- Human, Mouse
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- 详细信息
- 技术资料
- 抗体名：
B-raf Antibody抗体
- 抗体英文名：
B-raf Antibody
- 靶点：
BRAF
- 浓度：
1 mg/mL
- 应用范围：
ELISA, IF, IHC-P, WB
- 适应物种：
Human, Mouse
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1239042
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
Anti-B-raf antibody (orb1239042) was raised against a peptide corresponding to 19 amino acids near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf.
- 规格：
0.02 mg
产品描述：B-raf Antibody
别名:B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf
免疫原：Anti-B-raf antibody (orb1239042) was raised against a peptide corresponding to 19 amino acids near the center of human B-raf. The 免疫原 is located within amino acids 330 - 380 of B-raf.
预测反应性:Gallus, Rat
分子量:Predicted: 94kDObserved: 94 kD
防腐剂:B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
纯化:B-raf Antibody is affinity chromatography purified via peptide column.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:P15056
UniProt ID:P15056
Note:For research use only.
别名:B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf
免疫原：Anti-B-raf antibody (orb1239042) was raised against a peptide corresponding to 19 amino acids near the center of human B-raf. The 免疫原 is located within amino acids 330 - 380 of B-raf.
预测反应性:Gallus, Rat
分子量:Predicted: 94kDObserved: 94 kD
防腐剂:B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
纯化:B-raf Antibody is affinity chromatography purified via peptide column.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:P15056
UniProt ID:P15056
Note:For research use only.
Western Blot Validation in Human Brain Tissue. Loading: 15 µg of lysates per lane. Antibodies: B-raf orb1239042, (A: 1 µg/mL; B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: B-raf orb1240089, (0.5 µg/mL), B-raf orb1239042, (0.5 µg/mL) and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken (for orb1240089) and goat anti-rabbit (for orb1239042) IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of B-raf in Human Small Intestine Tissue. Immunohistochemical analysis of paraffin-embedded Human Small Intestine Tissue using anti-B-raf antibody (orb1239042) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of B-raf in Human Small Intestine Tissue. Immunofluorescent analysis of 4% Fixative-fixed Human Small Intestine Tissue labeling B-raf with orb1239042 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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B-raf Antibody抗体，orb1239042，biorbyt
￥3770
