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ASC Antibody抗体，orb1239217，bior

byt
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  • ￥3770
  • biorbyt已认证
  • orb1239217
  • 英国
  • 2025年12月08日
  • ELISA, ICC, IHC-P, WB
  • Human
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武汉博欧特生物科技有限公司
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    • 抗体名：

      ASC Antibody抗体

    • 抗体英文名：

      ASC Antibody

    • 靶点：

      PYCARD

    • 浓度：

      1 mg/mL

    • 应用范围：

      ELISA, ICC, IHC-P, WB

    • 适应物种：

      Human

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb1239217

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      Anti-ASC antibody (orb1239217) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ASC. The immunogen is located within the last 50 amino acids of ASC.

    • 规格：

      0.02 mg

    产品描述：ASC Antibody

    别名:ASC Antibody: ASC, TMS, TMS1, CARD5, TMS-1, ASC, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, hASC

    免疫原：Anti-ASC antibody (orb1239217) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ASC. The 免疫原 is located within the last 50 amino acids of ASC.

    预测反应性:Mouse, Rat

    分子量:Predicted: 22kDObserved: 22 kD

    防腐剂:ASC Antibody is supplied in PBS containing 0.02% sodium azide.

    纯化:ASC Antibody is affinity chromatography purified via peptide column.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    NCBI:BAA87339

    UniProt ID:Q9ULZ3

    Note:For research use only.

    产品细节图片1
    Western Blot Validation in Human HL60 Cells. Loading: 15 µg of lysates per lane. Antibodies: ASC orb1239217, (1 µg/mL) in the absence (A) or presence (B) of blocking peptide, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

    产品细节图片2
    Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: ASC orb1239217, (2 µg/mL), ASC, (2 µg/mL), beta-actin (1 µg/mL) and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit or goat anti-mouse (for ASC 39001) IgG HRP conjugate at 1:10000 dilution.

    产品细节图片3
    Western Blot Validation in Human THP-1 Cells. Loading: 15 µg of lysate per lane. Antibodies: ASC orb1239217, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

    产品细节图片4
    Immunohistochemistry Validation of ASC in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ASC antibody (orb1239217) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    产品细节图片5
    Immunofluorescence Validation of ASC in Human Spleen Tissue. Immunofluorescent analysis of 4% Fixative-fixed Human Spleen Tissue labeling ASC with orb1239217 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

    产品细节图片6
    Immunocytochemistry Validation of ASC in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-ASC antibody (orb1239217) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    产品细节图片7
    KD Validation of ASC in THP-1 Cells (Dowds et al., 2004). Immunofluorescence analysis with anti-ASC antibodies (orb1239217) was performed for BIM in 293 cells transfected with GFP siRNA or ASC siRNA. ASC expression was disrupted after ASC siRNA knockdown.

    产品细节图片8
    Overexpression Validation of ASC in HEK293T Cells (Dowds et al., 2004). Western blot analysis with anti-ASC antibodies (orb1239217) was performed for ASC in HEK293T cells transfected with pcDNA3-ASC.

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