- ￥11700
- Biorbyt
- orb1258890
- 英国
- 2026年07月29日
- IF, IHC, IP, WB
- Rabbit
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
DNMT1 Antibody 抗体
- 抗体英文名：
DNMT1 Antibody
- 靶点：
DNMT1
- 浓度：
batch dependent
- 应用范围：
IF, IHC, IP, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1258890
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
详询
- 规格：
100 ul
类别: Antibodies
产品子类型: Primary Antibody
描述: DNMT1 Antibody
别名: DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC9, DNMT, FLJ16293, MCMT, MGC104992, DNA methyltransferase 1, AIM
应用说明: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
免疫原: Recombinant protein of human DNMT1.
分子量: Observed: 200kDa
防腐剂: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
纯化方法: Affinity purification
研究领域: Epigenetics & Chromatin
UniProt ID: P26358
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: DNMT1 Antibody
别名: DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC9, DNMT, FLJ16293, MCMT, MGC104992, DNA methyltransferase 1, AIM
应用说明: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
免疫原: Recombinant protein of human DNMT1.
分子量: Observed: 200kDa
防腐剂: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
纯化方法: Affinity purification
研究领域: Epigenetics & Chromatin
UniProt ID: P26358
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Western blot analysis of extracts of various cell lines, using DNMT1 antibody (orb1258890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human breast cancer using DNMT1 antibody (orb1258890) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (orb1258890) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using DNMT1 antibody (orb1258890) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using DNMT1 antibody (orb1258890) at dilution of 1:100. Blue: DAPI for nuclear staining.
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文献和实验相关实验
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) (Abcam, Cambridge, UK; Sigma, St. Louis, USA.; Roche, Mannheim, Germany). We recently generated a GFP-binding protein (GBP) based on a single domain antibody derived from Lama alpaca (Rothbauer et al., 2008). This GFP-binding protein is characterized
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DNMT1 Antibody 抗体，orb1258890，Biorbyt
￥11700
