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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
PSME3 Antibody抗体
- 抗体英文名:
PSME3 Antibody
- 靶点:
PSME3
- 浓度:
batch dependent
- 应用范围:
IF, WB
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb1260785
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PSME3 (NP_005780.2).
- 规格:
100 ul
产品描述:PSME3 Antibody
别名:PSME3, Ki, PA28-gamma, PA28G, REG-GAMMA, HEL-S-283, PA28gamma
免疫原:A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PSME3 (NP_005780.2).
分子量:Observed: 35kDa
应用注释:WB: 1:1000 - 1:2000IF: 1:50 - 1:200
防腐剂:Liquid
纯化:Affinity 纯化
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P61289
Note:For research use only.




别名:PSME3, Ki, PA28-gamma, PA28G, REG-GAMMA, HEL-S-283, PA28gamma
免疫原:A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PSME3 (NP_005780.2).
分子量:Observed: 35kDa
应用注释:WB: 1:1000 - 1:2000IF: 1:50 - 1:200
防腐剂:Liquid
纯化:Affinity 纯化
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P61289
Note:For research use only.

Western blot analysis of extracts of various cell lines, using PSME3 antibody (orb1260785) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of C6 cells using PSME3 Polyclonal Antibody (orb1260785) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using PSME3 Polyclonal Antibody (orb1260785) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using PSME3 Polyclonal Antibody (orb1260785) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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文献和实验相关实验
「体温低」竟能更长寿!Nature Aging 研究揭示低温延寿的关键机制
线虫(Caenorhabditis elegans)中,低温(15℃)能促进激活因子 PSME-3 介导的蛋白酶活性,而且 PSME-3 的表达能延长寿命。研究还表明,将体外培养的人类细胞暴露在适度偏低的体温(36℃)下,能激活人类版本的 PSME-3,并有望减少疾病相关的蛋白质改变。 图片来源:Nature Aging 主要研究内容 寒冷诱导的 PA28γ/PSME3 触发蛋白酶体活性 蛋白酶体(一类蛋白质复合物)可以通过降解不需要的、损坏的和错误折叠的易于聚集的蛋白质,包括与疾病相关的突变蛋白,来预防衰老和病理
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PSME3 Antibody抗体,orb1260785,biorbyt
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