- ￥10400
- biorbyt
- orb1261271
- 英国
- 2025年12月08日
- ChIP, IF, IHC, IP, WB
- Human, Monkey, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
CTCF Antibody抗体
- 抗体英文名：
CTCF Antibody
- 靶点：
CTCF
- 浓度：
batch dependent
- 应用范围：
ChIP, IF, IHC, IP, WB
- 适应物种：
Human, Monkey, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1261271
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
- 规格：
100 ul
产品描述：CTCF Antibody
别名:CTCF, MRD21
免疫原：Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
分子量:Observed: 140kDa
应用注释：WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100ChIP: 1:50 - 1:200
防腐剂:Liquid
纯化:Affinity 纯化
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P49711
Note:For research use only.
别名:CTCF, MRD21
免疫原：Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
分子量:Observed: 140kDa
应用注释：WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100ChIP: 1:50 - 1:200
防腐剂:Liquid
纯化:Affinity 纯化
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P49711
Note:For research use only.
Western blot analysis of extracts of various cell lines, using CTCF antibody (orb1261271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human colon using CTCF antibody (orb1261271) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using CTCF antibody (orb1261271) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CTCF antibody (orb1261271) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CTCF antibody (orb1261271) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug CTCF antibody (orb1261271). Western blot was performed from the immunoprecipitate using CTCF antibody (orb1261271) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using CTCF antibody (orb1261271) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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CTCF Antibody抗体，orb1261271，biorbyt
￥10400
