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MyoD1 Antibody抗体,orb1269465,bi

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  • ¥11440
  • biorbyt已认证
  • orb1269465
  • 英国
  • 2025年12月07日
  • IF, WB
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      MyoD1 Antibody抗体

    • 抗体英文名

      MyoD1 Antibody

    • 靶点

      MYOD1

    • 浓度

      batch dependent

    • 应用范围

      IF, WB

    • 适应物种

      Human

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb1269465

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      Rabbit Ig

    • 免疫原

      This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1.

    • 规格

      400 ul

    产品描述:MyoD1 Antibody

    别名:Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3, MYOD1, BHLHC1, MYF3, MYOD

    免疫原:This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1.

    分子量:35 kDa

    应用注释:For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50

    防腐剂:Supplied in PBS with 0.09% (W/V) sodium azide.

    纯化:This antibody is purified through a protein A column, followed by peptide affinity 纯化.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    NCBI:P15172

    UniProt ID:P15172

    Note:For research use only.

    产品细节图片1
    Western Blot at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: RD whole cell lysate Lysates/proteins at 20 ug per lane.

    产品细节图片2
    Western blot analysis in Hela cell line and human placenta tissue lysates (35 ug/lane).

    产品细节图片3
    Fluorescent confocal image of Hela cell stained with MyoD1 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MyoD1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MyoD1 immunoreactivity is localized to nucleus significantly.

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    图标文献和实验
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