- ￥10400
- biorbyt
- orb1274540
- 英国
- 2025年12月07日
- IF, IHC, IP, WB
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
EGFR Antibody, KO Validated抗体
- 抗体英文名：
EGFR Antibody, KO Validated
- 靶点：
EGFR
- 浓度：
batch dependent
- 应用范围：
IF, IHC, IP, WB
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1274540
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
- 规格：
100 ul
产品描述：EGFR Antibody, KO Validated
别名:Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
免疫原：Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
分子量:Observed: 175kDa
应用注释：WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
防腐剂:Liquid
纯化:Affinity 纯化
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P00533
Note:For research use only.
别名:Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
免疫原：Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
分子量:Observed: 175kDa
应用注释：WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
防腐剂:Liquid
纯化:Affinity 纯化
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P00533
Note:For research use only.
Western blot analysis of extracts of OVCAR3 cells, using EGFR antibody (orb1274540) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (orb1274540) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody (orb1274540) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody (orb1274540) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using EGFR antibody (orb1274540) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody (orb1274540) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using EGFR antibody (orb1274540) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug EGFR antibody (orb1274540). Western blot was performed from the immunoprecipitate using EGFR antibody (orb1274540) at a dilution of 1:1000.
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EGFR Antibody, KO Validated抗体，orb1274540，biorbyt
￥10400
