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NF-L Rabbit Polyclonal Antibod

y抗体，orb1152008，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb1152008
  • 英国
  • 2025年12月07日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      NF-L Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      NF-L Rabbit Polyclonal Antibody

    • 靶点：

      NEFL

    • 浓度：

      1mg/ml

    • 应用范围：

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb1152008

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from human NH-L intermedial (301-400/543aa)

    • 规格：

      50 ul

    产品描述：NF-L Rabbit Polyclonal Antibody

    别名:Neurofilament L; Neurofilament 68; Neurofilament triplet L; 70 kD Neurofilament Light; 68kDa neurofilament protein; CMT 1F; CMT 2E; CMT1F; CMT2E; FLJ53642; Light molecular weight neurofilament protein; NEFL; Neurofilament light; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament protein, light chain; Neurofilament subunit NF L; Neurofilament triplet L protein; NF 68; NF L; NF68; NFL; NFL_HUMAN.

    免疫原：KLH conjugated synthetic peptide derived from human NH-L intermedial (301-400/543aa)

    预测反应性:Mouse, Rat

    分子量:68 kDa

    应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P07196

    Note:For research use only.

    产品细节图片1
    Blank control: SHSY5Y. Primary Antibody (green line): Rabbit Anti-NF-L antibody (orb1152008), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Human U251 cell lysates, Primary: Anti-NF-L (orb1152008) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 68 kDa.

    产品细节图片3
    Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: 0.4% Pepsin, 37°C, 30 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NF-L/Neurofilament L/Neurofilament 68 Polyclonal Antibody, Unconjugated (orb1152008) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

    产品细节图片4
    Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NF-L/Neurofilament L/Neurofilament 68 Polyclonal Antibody, Unconjugated (orb1152008) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

    产品细节图片5
    Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NF-L/Neurofilament L/Neurofilament 68 Polyclonal Antibody, Unconjugated (orb1152008) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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