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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
O52L2 rabbit pAb抗体
- 抗体英文名:
O52L2 rabbit pAb
- 浓度:
1 mg/ml
- 应用范围:
ELISA, WB
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb774551
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 亚型:
IgG
- 免疫原:
Synthesized peptide derived from human protein . at AA range: 190-270
- 规格:
50 ul
免疫原:Synthesized peptide derived from human protein . at AA range: 190-270
分子量:35
应用稀释比例:WB 1:500-2000 ELISA 1:5000-20000
防腐剂:PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
纯化:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
来源:Rabbit
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Note:For research use only.

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文献和实验我的石蜡切片又「团灭」了!没关系,专业技术人员与您并肩推塔!
Antibody Diluent #8112 稀释PD-L2抗体(#82723)稀释度为1:200。11. 横沥封闭液,用卫生纸尽量吸干封闭液后,将一抗滴加在切片上。(此步建议一张张完成,防止干片)12. 将湿盒小心移入4度冰箱,过夜。CST技术人员正在配置和滴加抗体二抗孵育与显色13. 小心取出湿盒,在室温平衡45分钟左右。14. 洗涤:切片浸没于TBST中,3 ×5 min。15. 将二抗SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)#8114
-biotin peroxidase(e.g rabbit anti antigen X, goat anti-rabbit biotin, avidin HRP) 1- Wash twice in TBS 0.05M pH7.5, to which 0.01% Tween 20 has been added. 2- Briefly blot
Immunohistochemistry on fixed, paraffin-embedded sections
: a) be absolutely flat, b) maintain moisture over 3 days without drying. c) be washable. Double indirect AP immunohistochemistry (e.g rabbit anti antigen X, goat anti-rabbit AP, rabbit anti-goat AP) 1- Wash twice in TBS 0.05M pH
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