- ￥3250
- biorbyt
- orb768829
- 英国
- 2025年12月07日
- ELISA, IHC-P
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
A-Raf (phospho Ser299) rabbit pAb抗体
- 抗体英文名：
A-Raf (phospho Ser299) rabbit pAb
- 浓度：
1 mg/ml
- 应用范围：
ELISA, IHC-P
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb768829
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 亚型：
IgG
- 免疫原：
The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Ser299. AA range:266-315
- 规格：
50 ul
别名:Anti-ARAF antibody, anti-ARAF1 antibody, anti-PKS antibody, anti-PKS2 antibody, anti-Serine/threonine-protein kinase A-Raf antibody, anti-Proto-oncogene A-Raf antibody, anti-Proto-oncogene A-Raf-1 antibody, anti-Proto-oncogene Pks antibody
免疫原：The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Ser299. AA range:266-315
分子量:68kD
应用稀释比例：Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
防腐剂:PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
纯化:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
来源:Rabbit
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Note:For research use only.
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文献和实验Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation
including proliferation, differentiation, and survival. In most of these cases, the ERK cascade is activated downstream of the small GTPase Ras that, upon activation, recruits and activates the first tier in the cascade, which contains the Raf kinases
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
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