p53 Rabbit pAb Antibody 抗体,orb765994,Biorbyt产品图

p53 Rabbit pAb Antibody 抗体,orb

765994,Biorbyt
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  • ¥6669
  • Biorbyt已认证
  • orb765994
  • 英国
  • 2026年07月24日
  • ELISA, IF, IHC, KO/KD Validated, WB
  • Human, Monkey, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      p53 Rabbit pAb Antibody 抗体

    • 抗体英文名:

      p53 Rabbit pAb Antibody

    • 浓度:

      1 mg/ml

    • 应用范围:

      ELISA, IF, IHC, KO/KD Validated, WB

    • 适应物种:

      Human, Monkey, Mouse, Rat

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb765994

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 亚型:

      IgG

    • 免疫原:

      详询

    • 规格:

      100 ul

    类别: Antibodies

    描述: Rabbit polyclonal antibody to p53

    别名: Anti-TP53 antibody, anti-P53 antibody, anti-Cellular tumor antigen p53 antibody, anti-Antigen NY-CO-13 antibody, anti-Phosphoprotein p53 antibody, anti-Tumor suppressor p53 antibody

    应用稀释比例: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

    免疫原: The antiserum was produced against synthesized peptide derived from human p53. AA range:10-59

    分子量: 44

    防腐剂: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

    纯化方法: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    来源: Rabbit

    研究领域: Cancer Biology

    UniProt ID: P04637

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of lysates from 1) p53 knockout A431 cell, 2) A431 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1 hour.

    产品细节图片2
    Immunofluorescence analysis of human-liver tissue. 1, p53 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

    产品细节图片3
    Immunofluorescence analysis of human-liver tissue. 1, p53 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

    产品细节图片4
    Immunofluorescence analysis of human-lung tissue. 1, p53 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

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    图标文献和实验
    相关实验
    • secondary antibody review -- data from 99 publications

      cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

    • Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

      = NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific

    • 类器官多维鉴定(基因测序等)

      -mouse-mab.html 可用于乳腺癌组织的免疫组化分析 Ki67 Antidiv (YA322) (HY-P80506) www.medchemexpress.cn/antibody/ki67-rabbit-mab.html 可用于乳腺癌组织的免疫组化分析 p53 Antidiv (YA250) (HY-P80257) www.medchemexpress.cn/antibody/p53-rabbit-mab.html 可用于乳腺癌组织的免疫组化分析 Cytokeratin 7 Antidiv 

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