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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Progesterone/P, BSA conjugated
- 供应商:
武汉益普生物科技有限公司
- 规格:
100μg/1mg
| 规格: | 100μg | 产品价格: | ¥1200.0 |
|---|---|---|---|
| 规格: | 1mg | 产品价格: | ¥6000.0 |
| 产品名 | Progesterone/P, BSA conjugated |
| 货号 | SGK61501 |
| 别名 | P4; Pregn-4-ene-3,20-dione; Corpus Luteum Hormone; Luteosterone; Progestin; Pregnenedione |
| Accession号 | 57-83-0 |
| 种属 | General |
| 内毒素水平 | Please contact with the lab for this information. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |

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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
ELISA PROTOCOL 【Yale University 】
1 hour at room temperature.4) Discard the primary antibody solution and wash 3X with TST.5) Add 50 µl of diluted secondary antibody conjugated to AlkalinePhosphatase. I use a 1:500 dilution of rabbit anti-mouse IgG
ELISA (Enzyme-Linked ImmunoadSorbent Assa
is from colourless to blue (yellow when acid is added). 2) Coat plate with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C. 3) Remove antigen and wash 3x with 1x PBS. 4) Block with 1% BSA (100
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