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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Biotinylated Human CD269/TNFRSF17/BCMA Protein, C-Fc-Avi
- 供应商:
武汉益普生物科技有限公司
- 规格:
20μg/100μg
| 规格: | 20μg | 产品价格: | ¥2160.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥5700.0 |
| 产品名 | Biotinylated Human CD269/TNFRSF17/BCMA Protein, C-Fc-Avi |
| 货号 | AHF92401 |
| 别名 | Tumor necrosis factor receptor superfamily member 17, TNFRSF17, B-cell maturation protein, CD269 |
| 表达系统 | Mammalian Cells |
| Accession号 | Q02223 |
| 种属 | Homo sapiens (Human) |
| 标记 | Biotin |
| 蛋白长度 | Met1-Ala54 |
| 预测分子量 | 34.6 kDa |
| 性质 | Recombinant |
| 内毒素水平 | < 1 EU/µg as determined by LAL test. |
| 纯度 | > 90% as determined by SDS-PAGE. |
| 应用 | Bioactivity, ELISA, Immunogen, SDS-PAGE, WB |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |

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文献和实验【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
., Finstad, J., Williams, Jr., R. C. 1970. Phylogenetic insight into evolution of mammalian Fc fragment of g G globulin using staphylococcal protein A. J. Immunol. 104:140-147. Kronvall, G. 1973. A surface component in group A, C, and G streptococci
on the beads was detected using biotinylated antigen/streptavidin-phycoerythrin. The method was demonstrated by isotyping antibodies directed against an anti-CD11a antibody therapeutic (RAPTIVA� /efalizumab) from the serum of a cynomolgus monkey hyper-immunized
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