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AF-10 rabbit pAb抗体,orb764484,b

iorbyt
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  • ¥3250
  • biorbyt已认证
  • orb764484
  • 英国
  • 2025年12月05日
  • ELISA, IF, IHC-P, WB
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      AF-10 rabbit pAb抗体

    • 抗体英文名

      AF-10 rabbit pAb

    • 浓度

      1 mg/ml

    • 应用范围

      ELISA, IF, IHC-P, WB

    • 适应物种

      Human, Mouse

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb764484

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 亚型

      IgG

    • 免疫原

      The antiserum was produced against synthesized peptide derived from human AF10. AA range:211-260

    • 规格

      50 ul

    产品描述:Rabbit polyclonal antibody to AF-10

    别名:Anti-MLLT10 antibody, anti-AF10 antibody, anti-Protein AF-10 antibody, anti-ALL1-fused gene from chromosome 10 protein antibody

    免疫原:The antiserum was produced against synthesized peptide derived from human AF10. AA range:211-260

    分子量:109

    应用稀释比例:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

    防腐剂:PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

    纯化:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    来源:Rabbit

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Note:For research use only.

    产品细节图片1
    Immunofluorescence analysis of Hela cell. 1, AF-10 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). COX IV Monoclonal Antibody (6C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

    产品细节图片2
    Western Blot analysis of various cells using AF-10 Polyclonal Antibody diluted at 1:1000.

    产品细节图片3
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

    产品细节图片4
    Western blot analysis of lysates from COLO205 and Jurkat cells, using AF10 Antibody. The lane on the right is blocked with the synthesized peptide.

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