- ￥3250
- biorbyt
- orb764208
- 英国
- 2025年12月05日
- ELISA, WB
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
ICAM-1 (phospho Tyr512) rabbit pAb抗体
- 抗体英文名：
ICAM-1 (phospho Tyr512) rabbit pAb
- 浓度：
1 mg/ml
- 应用范围：
ELISA, WB
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb764208
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 亚型：
IgG
- 免疫原：
The antiserum was produced against synthesized peptide derived from human ICAM-1 around the phosphorylation site of Tyr512. AA range:479-528
- 规格：
50 ul
别名:Anti-ICAM1 antibody, anti-Intercellular adhesion molecule 1 antibody, anti-ICAM-1 antibody, anti-Major group rhinovirus receptor antibody, anti-CD antigen CD54 antibody
免疫原：The antiserum was produced against synthesized peptide derived from human ICAM-1 around the phosphorylation site of Tyr512. AA range:479-528
分子量:57
应用稀释比例：Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
防腐剂:PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
纯化:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
来源:Rabbit
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Note:For research use only.
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文献和实验ELISA FOR DETECTING SECRETED ICAM-1
. Add 50 ul of culture supernatant containing secreted ICAM-1. Include positive control wells of purified ICAM-1 ranging from 512 ng/ml to 2 ng/ml. Incubate 30 min at 37o C. Wash 4X with PBS. Add 50 ul/well Biotinylated R6.5 diluted
SANDWICH ELISA FOR DETECTING SECRETED ICAM-1
. Add 50 ul of culture supernatant containing secreted ICAM-1. Include positive control wells of purified ICAM-1 ranging from 512 ng/ml to 2 ng/ml. Incubate 30 min at 37o C. Wash 4X with PBS. Add 50 ul/well Biotinylated R6.5 diluted
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
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