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RAB3D Antibody (FITC)抗体,orb751

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  • ¥9230
  • biorbyt已认证
  • orb751915
  • 英国
  • 2025年12月05日
  • WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      RAB3D Antibody (FITC)抗体

    • 抗体英文名

      RAB3D Antibody (FITC)

    • 靶点

      RAB3D

    • 浓度

      1mg/ml

    • 应用范围

      WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb751915

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      FITC

    • 克隆性

      Monoclonal

    • 亚型

      IgG1

    • 免疫原

      Full length recombinant RAB3D protein

    • 规格

      100 ug

    产品描述:Mouse monoclonal antibody against RAB3D conjugated to FITC

    别名:Member RAS Oncogene Family, D2-2, GOV, RAB16, RAD3D, Ras-Related Protein Rab-3D

    免疫原:Full length recombinant RAB3D protein

    克隆性:5000000000

    分子量:24 kDa

    应用稀释比例:WB (1:1000)

    应用注释:A 1:1000 dilution of SMC-614 was sufficient for detection of RAB3D in 10 µg of A549, T-47D cells, mouse and rat brain, kidney by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

    防腐剂:640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

    纯化:Protein G Purified

    保存说明:Conjugated antibodies should be stored according to the product label

    NCBI:NP_004274

    Entrez:9545

    UniProt ID:O95716

    Note:For research use only.

    产品细节图片1
    Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and RAB3D showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9. Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing RAB3D. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody.

    产品细节图片2
    Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9. Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney. Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

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    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • secondary antibody review -- data from 99 publications

      localization of tagged SAX-3 in 293T cell Molecular Probes 2       Alexa Fluor 647 immunocytochemistry   localization of BCG phagosomes   3       Alexa Fluor 647 immunohistochemistry   detect surface DFz2   3       FITC immunohistochemistry   determine

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