RAB3D Antibody (FITC) 抗体,orb751915,Biorbyt产品图

RAB3D Antibody (FITC) 抗体,orb75

1915,Biorbyt
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  • ¥8307
  • Biorbyt已认证
  • orb751915
  • 英国
  • 2026年07月28日
  • WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      RAB3D Antibody (FITC) 抗体

    • 抗体英文名:

      RAB3D Antibody (FITC)

    • 靶点:

      RAB3D

    • 浓度:

      1mg/ml

    • 应用范围:

      WB

    • 宿主:

      Mouse

    • 适应物种:

      Human, Mouse, Rat

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb751915

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      FITC

    • 克隆性:

      Monoclonal

    • 保存条件:

      详询

    • 亚型:

      IgG1

    • 免疫原:

      详询

    • 规格:

      100 ug

    类别: Antibodies

    描述: Mouse monoclonal antibody against RAB3D conjugated to FITC

    克隆号: 5000000000

    别名: Member RAS Oncogene Family, D2-2, GOV, RAB16, RAD3D, Ras-Related Protein Rab-3D

    应用稀释比例: WB (1:1000)

    应用说明: A 1:1000 dilution was sufficient for detection of RAB3D in 10 µg of A549, T-47D cells, mouse and rat brain, kidney by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

    免疫原: Full length recombinant RAB3D protein

    分子量: 24 kDa

    防腐剂: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

    纯化方法: Protein G Purified

    特异性: Detects ~24 kDa. Does not show cross reactivity to RAB3A, RAB3B, or RAB3C.

    研究领域: Signal Transduction

    UniProt ID: O95716

    NCBI 参考序列: NP_004274

    Entrez 基因 ID: 9545

    保存条件: Conjugated antibodies should be stored according to the product label

    备注: For research use only.

    产品细节图片1
    Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and RAB3D showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9. Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing RAB3D. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody.

    产品细节图片2
    Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9. Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney. Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

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    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • secondary antibody review -- data from 99 publications

      localization of tagged SAX-3 in 293T cell Molecular Probes 2       Alexa Fluor 647 immunocytochemistry   localization of BCG phagosomes   3       Alexa Fluor 647 immunohistochemistry   detect surface DFz2   3       FITC immunohistochemistry   determine

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