Phospho-Progesterone Receptor (Ser190) Rabbit Polyclonal Antibody抗体,orb704511,biorbyt

Phospho-Progesterone Receptor

(Ser190) Rabbit Polyclonal Antibody抗体,orb704511,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb704511
  • 英国
  • 2025年12月05日
  • FC, WB
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      Phospho-Progesterone Receptor (Ser190) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-Progesterone Receptor (Ser190) Rabbit Polyclonal Antibody

    • 靶点

      PGR

    • 浓度

      1mg/ml

    • 应用范围

      FC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb704511

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from rat Progesterone Receptor around the phosphorylation site of Ser190 GL(p-S)P

    • 规格

      50 ul

    产品描述:Phospho-Progesterone Receptor (Ser190) Rabbit Polyclonal Antibody

    别名:PGR(phospho S190); NR3C3; Nuclear receptor subfamily 3 group C member 3; PGR; PR; PRA; PRB; Progesterone receptor; Progestin receptor form A; Progestin receptor form B; PRGR_RAT.

    免疫原:KLH conjugated Synthesised phosphopeptide derived from rat Progesterone Receptor around the phosphorylation site of Ser190 GL(p-S)P

    预测反应性:Bovine, Mouse

    分子量:99 kDa

    应用稀释比例:WB=1:500-2000, Flow-Cyt=1ug/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q63449

    Note:For research use only.

    Phospho-Progesterone Receptor
    Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Phospho-Progesterone Receptor (Ser190) antibody (orb704511), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    Phospho-Progesterone Receptor
    Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Progesterone Receptor (Ser190) (orb704511) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD.

    Phospho-Progesterone Receptor
    Sample: Lane 1: Uterus (Mouse) Lysate at 40 ug, Lane 2: Testis (Mouse) Lysate at 40 ug, Lane 3: Testis (Rat) Lysate at 40 ug, Lane 4: MCF-7 (Human) Cell Lysate at 30 ug, Lane 5: HepG2 (Human) Cell Lysate at 30 ug, Lane 6: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-progesterone receptor (orb704511) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 118/82-88 kD, Observed band size: 120 kD.

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