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KDM1/LSD1 Rabbit Polyclonal An

tibody抗体,orb704510,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb704510
  • 英国
  • 2025年12月05日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      KDM1/LSD1 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      KDM1/LSD1 Rabbit Polyclonal Antibody

    • 靶点

      KDM1A

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb704510

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human KDM1 (101-200/852aa)

    • 规格

      50 ul

    产品描述:KDM1/LSD1 Rabbit Polyclonal Antibody

    别名:Amine oxidase(flavin containing) domain 2; Amine oxidase flavin containing domain 2; Amine oxidase flavin containing domain protein 2; AOF 2; AOF2; BHC110; BRAF35 HDAC complex protein BHC110; BRAF35-HDAC complex protein BHC110; FAD binding protein BRAF35 HDAC complex, 110 kDa subunit; Flavin containing amine oxidase domain containing protein 2; Flavin-containing amine oxidase domain-containing protein 2; KDM 1; Kdm1a; KDM1A_HUMAN; KIAA0601; LSD 1; LSD1; Lysine(K) specific demethylase 1; Lysine(K) specific demethylase 1A; Lysine specific histone demethylase 1; Lysine specific histone demethylase 1A; Lysine-specific histone demethylase 1; Lysine-specific histone demethylase 1A.

    免疫原:KLH conjugated synthetic peptide derived from human KDM1 (101-200/852aa)

    预测反应性:Bovine, Canine, Gallus, Porcine, Rabbit

    分子量:94 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:O60341

    Note:For research use only.

    产品细节图片1
    Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-KDM1/LSD1 antibody (orb704510), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (KDM1/LSD1) Polyclonal Antibody, Unconjugated (orb704510) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: 293T (Human) Cell Lysate at 30 ug, Lane 4: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-KDM1/LSD1 (orb704510) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

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    图标文献和实验
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