- ￥3120
- biorbyt
- orb704426
- 英国
- 2025年12月05日
- FC, WB
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
CD144/VE Cadherin Rabbit Polyclonal Antibody抗体
- 抗体英文名：
CD144/VE Cadherin Rabbit Polyclonal Antibody
- 靶点：
CDH5
- 浓度：
1mg/ml
- 应用范围：
FC, WB
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb704426
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from mouse CD144/VE Cadherin (46-140/784aa)
- 规格：
50 ul
产品描述：CD144/VE Cadherin Rabbit Polyclonal Antibody
别名:VE-cadherin; 7B4 antigen; VECD; Vascular endothelial cell cadherin; 7B 4; 7B4; 7B4 antigen; Cadherin 5; Cadherin 5 type 2; Cadherin-5; Cadherin5; CD 144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Vascular endothelial cadherin; VE Cad antibody VEC; CADH5_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from mouse CD144/VE Cadherin (46-140/784aa)
预测反应性:Human
分子量:86 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=1ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P55284
Note:For research use only.
别名:VE-cadherin; 7B4 antigen; VECD; Vascular endothelial cell cadherin; 7B 4; 7B4; 7B4 antigen; Cadherin 5; Cadherin 5 type 2; Cadherin-5; Cadherin5; CD 144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Vascular endothelial cadherin; VE Cad antibody VEC; CADH5_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from mouse CD144/VE Cadherin (46-140/784aa)
预测反应性:Human
分子量:86 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=1ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P55284
Note:For research use only.
Blank control: HUVC. Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (orb704426), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (orb704426), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human HUVEC cell lysates, Lane 3: Human A431 cell lysates, Lane 4: Human HeLa cell lysates, Primary: Anti-CD144/VE Cadherin (orb704426) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 86 kDa, Observed band size: 140 kDa.
Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: HUVEC (Human) Cell Lysate at 30 ug, Lane 3: A431 (Human) Cell Lysate at 30 ug, Lane 4: LOVO (Human) Cell Lysate at 30 ug, Lane 5: SiHa (Human) Cell Lysate at 30 ug, Primary: Anti-CD144/VE Cadherin (orb704426) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.
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文献和实验相关实验
of First Hospital of Harbin Medical University. RPMI 1640 was purchased from GIBCO. Propidium iodide and Rnase were from Sigma Chemical Co. A murine monoclonal antibody against human Bcl-2 and Bax oncoprotein and antimi ce rabbit polyclonal antibody were purchased
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CD144/VE Cadherin Rabbit Polyclonal Antibody抗体，orb704426，biorbyt
￥3120
