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NF-kappaB p105/p50 Antibody抗体,

orb393122,biorbyt
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  • ¥1560
  • biorbyt已认证
  • orb393122
  • 英国
  • 2025年12月05日
  • IF, IHC, WB
  • Rabbit
  • Bovine, Canine, Gallus, Human, Mouse, Porcine, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      NF-kappaB p105/p50 Antibody抗体

    • 抗体英文名

      NF-kappaB p105/p50 Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Bovine, Canine, Gallus, Human, Mouse, Porcine, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb393122

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p105/p50. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The NF-kappaB p105/p50 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p105/p50. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p105/p50. The exact sequence is proprietary.

    应用稀释比例:WB: 1:500:1000, IHC-P: 1:100:200

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:4790, 18033

    UniProt ID:Q63369, P25799, P19838

    Note:For research use only.

    产品细节图片1
    Western blot analysis of NF-kappaB p105/p50 expression in MCF7 (A), U2OS (B), A375 (C), mouse muscle (D), mouse liver (E) whole cell lysates. (Predicted band size: 85; 105 kD; Observed band size: 105; 50 kD)

    产品细节图片2
    Immunohistochemical analysis of NF-kappaB p105/p50 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of NF-kappaB p105/p50 staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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    图标文献和实验
    相关实验
    • 【进展|热点】Nature Immunology:发现三个调控NF-kB非经典信号通路的microRNA

      ://www.nature.com/ni/journal/v11/n9/abs/ni.1918.html 内容简介: NF-κB是一组重要的转录因子,参与了诸多免疫反应过程,其中最为人熟知的就是在天然免疫中,NF-κB是最终启动炎症因子表达的因素之一。 NF-κB家族包括5个成员,即RelA(p65)、RelB、c-Rel、p105-p50(NF-κB1)和p100-p52(NF-κB2),两两形成同源或者异源二聚体的形式发挥作用。通常情况下,NF-κB与其抑制蛋白IκB(包括IκBα、IκBβ

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