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- 详细信息
- 技术资料
- 抗体名:
GABA A Receptor Antibody抗体
- 抗体英文名:
GABA A Receptor Antibody
- 靶点:
GABA A Receptor
- 浓度:
1 mg/ml
- 应用范围:
ICC, IF, IHC, WB
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb376696
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 亚型:
IgG1
- 免疫原:
Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
- 规格:
100 ug
产品描述:Mouse Anti-Rat GABA-A Receptor Alpha4 Monoclonal IgG1
别名:GABA A receptor subunit alpha 4, GABRA4, Gamma aminobutyric acid A receptor Alpha 4, Gamma-aminobutyric acid receptor subunit alpha-4, GBRA4_HUMAN
免疫原:Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
克隆性:N398A/34 (Formerly sold as S398A-34)
分子量:60kDa
应用稀释比例:WB (1:1000), ICC/IF (1:100)
应用注释:A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
防腐剂:PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
纯化:Protein G Purified
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:NP_542154.3
Entrez:140675
UniProt ID:P28471
Note:For research use only.


别名:GABA A receptor subunit alpha 4, GABRA4, Gamma aminobutyric acid A receptor Alpha 4, Gamma-aminobutyric acid receptor subunit alpha-4, GBRA4_HUMAN
免疫原:Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
克隆性:N398A/34 (Formerly sold as S398A-34)
分子量:60kDa
应用稀释比例:WB (1:1000), ICC/IF (1:100)
应用注释:A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
防腐剂:PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
纯化:Protein G Purified
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:NP_542154.3
Entrez:140675
UniProt ID:P28471
Note:For research use only.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody, Clone N398A/34. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.

Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone N398A/34. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 μg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.
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GABA A Receptor Antibody抗体,orb376696,biorbyt
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