FLAG-tag Antibody抗体,orb323042,biorbyt

FLAG-tag Antibody抗体,orb323042,

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  • ¥1300
  • biorbyt已认证
  • orb323042
  • 英国
  • 2025年12月05日
  • IF, IP, WB
  • Mouse
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    • 详细信息
    • 技术资料
    • 抗体名

      FLAG-tag Antibody抗体

    • 抗体英文名

      FLAG-tag Antibody

    • 应用范围

      IF, IP, WB

    • 宿主

      Mouse

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb323042

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Monoclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence of FLAG-tag. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The FLAG-tag Antibody is suitable for IF, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of FLAG-tag. The exact sequence is proprietary. Purification: Affinity chromatography

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence of FLAG-tag. The exact sequence is proprietary.

    应用稀释比例:WB: 1:2000-1:5000, IF/ICC: 1:200-1:500, IP: 1:100-1:200

    纯化:Affinity chromatography

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Note:For research use only.

    FLAG-tag Antibody抗体,orb323042,
    Western blot analysis of over-expressed FLAG-tagged protein in 293T cell lysate. (Predicted band size: \; Observed band size: \)

    FLAG-tag Antibody抗体,orb323042,
    Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

    FLAG-tag Antibody抗体,orb323042,
    Immunoprecipitation of FLAG-tagged protein from HEK293T cells transfected with vector overexpressing Flag tag, using Anti-FLAG-tag Antibody.

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