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p53 (Acetyl-K381) Antibody抗体,o

rb304643,biorbyt
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  • ¥1820
  • biorbyt已认证
  • orb304643
  • 英国
  • 2025年12月05日
  • IF, IHC, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      p53 (Acetyl-K381) Antibody抗体

    • 抗体英文名

      p53 (Acetyl-K381) Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb304643

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K381 of human p53 protein. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The p53 (Acetyl-K381) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K381 of human p53 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

    免疫原:KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K381 of human p53 protein. The exact sequence is proprietary.

    应用稀释比例:WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:7157

    UniProt ID:P04637

    Note:For research use only.

    产品细节图片1
    Western blot analysis of p53 (Acetyl-K381) expression in HEK293T (A), LO2 (B) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 53 kD)

    产品细节图片2
    Immunohistochemical analysis of p53 (Acetyl-K381) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of p53 (Acetyl-K381) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    图标文献和实验
    相关实验
    • 细胞自噬系统介绍

      modulator) 相关文章:DRAM, a p53-Induced Modulator of Autophagy, Is Critical for Apoptosis.pdf http://www.namipan.com/d/69fee934467a3fc4b9a70b88c9292160a631c78f42111300 4)TP53INP2 (Tumour Protein 53 Induced Nuclear Protein 2) 相关文章:The TP

    • secondary antibody review -- data from 99 publications

      cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

    •  白细胞分化抗原

      -sylphosphatidylinositol,GPI)连接方式“锚”在细胞膜上。少数白细胞分化抗原是碳水化合物半抗原。白细胞分化抗原参与机体重要的生理和病理过程。假如:(1)免疫应答过程中免疫细胞的相互识别,免疫细胞抗原识别、活化、增殖和分化,免疫效应功能的发挥;(2)造血细胞的分化和造血过程的调控;(3)炎症发生;(4)细胞的迁移如肿瘤细胞的转移等。 第一节 白细胞分化抗原的分类   80年代初以来,由于单克隆抗体,分子克隆、基因转染细胞系等技术在白细胞分化抗原研究中得到广泛深入的应用,有关白细胞分化抗原的研究

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