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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Goat Human IgG Fab - FITC Antibody抗体
- 抗体英文名:
Goat Human IgG Fab - FITC Antibody
- 应用范围:
ELISA, FC, IF, IHC
- 宿主:
Goat
- 适应物种:
Human
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb216346
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
FITC
- 克隆性:
Polyclonal
- 形态:
1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% rAlbumin, 50% glycerol, 0.05% Sodium Azide
- 免疫原:
Human IgG Fab
- 规格:
100 ul
免疫原:Human IgG Fab
应用稀释比例:IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)
纯化:The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
保存说明:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:For research use only.
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文献和实验【摘要】目的探讨嵌合抗CD20抗体Fab’片段的抗肿瘤机制。方法应用MTT法检测Fab’片段对Raji细胞生长的抑制作用,用Annexin V-FITC和PI测定Fab’片段对Raji细胞凋亡的诱导作用,用RT-PCR和Western blot检测Raji细胞中bcl-2表达的变化。结果嵌合抗CD20Fab’片段对Raji细胞具有明显的抑制作用IC50值为24.2 µg/ml,并呈剂量依赖性;嵌合抗CD20Fab’片段能诱导Raji细胞的凋亡,并降低Raji细胞中bcl-2基因及其蛋白的表达
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Immunohistochemisty-Fluorescence Protocol
antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted 1:500) in Wash Buffer-Saponin. Wash the cells twice in Wash Buffer-Saponin. Incubate the centrifuged cell pellet
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