- ￥1170
- biorbyt
- orb216263
- 英国
- 2025年12月05日
- ELISA, IHC, WB
- Mouse
- Porcine
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Mouse Pig IgG (H&L) Antibody抗体
- 抗体英文名：
Mouse Pig IgG (H&L) Antibody
- 应用范围：
ELISA, IHC, WB
- 宿主：
Mouse
- 适应物种：
Porcine
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb216263
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 形态：
1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, 0.05% Sodium Azide
- 免疫原：
Pig IgG
- 规格：
100 ul
免疫原：Pig IgG
应用稀释比例：ELISA: 1:5000-1:20000, WB: 1:5000-1:20000, IHC-P: 1:500-1:1000
纯化:The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Note:For research use only.
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
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