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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Rabbit Sheep IgG (H&L) Antibody抗体
- 抗体英文名:
Rabbit Sheep IgG (H&L) Antibody
- 应用范围:
ELISA, IHC, WB
- 宿主:
Rabbit
- 适应物种:
Sheep
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb216250
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, 0.05% Sodium Azide
- 免疫原:
Sheep IgG
- 规格:
100 ul
免疫原:Sheep IgG
应用稀释比例:ELISA: 1:5000-1:20000, WB: 1:5000-1:20000, IHC-P: 1:500-1:1000
纯化:The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Note:For research use only.
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
To immunostain using enzyme-coupled secondary antibodies: i. Rinse slides in PBS and remove excess solution. ii. Add 40 µL of secondary antibody (e.g., anti-rabbit IgG [Fc] HRP conjugate, 1:100 dilution
/ml 2 ml 10 ml 3055 8970 112.03 112.04 Dynabeads M-280 Sheep anti-Rabbit IgG, 6.6x108/ml 2 ml 10 ml 3055 8970 3、Dynabeads DNA
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
of antibody or control (normal rabbit serum, mouse isotype control,, etc.) to each tube and incubate overnight for 6 h at room temperature. 7) Add 100 µl of Protein-A-Sepharose solution (shake to suspend slurry before pipetting) and incubate on a tube
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