- ￥1820
- biorbyt
- orb214215
- 英国
- 2025年12月05日
- IF, IHC, WB
- Rabbit
- Gallus, Human, Monkey, Mouse, Porcine, Rat, Sheep
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- 详细信息
- 技术资料
- 抗体名：
SMAD3 (Phospho-S204) Antibody抗体
- 抗体英文名：
SMAD3 (Phospho-S204) Antibody
- 应用范围：
IF, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Gallus, Human, Monkey, Mouse, Porcine, Rat, Sheep
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb214215
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原：
KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S204 of human SMAD3 protein. The exact sequence is proprietary.
- 规格：
30 ul
产品描述：The SMAD3 (Phospho-S204) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S204 of human SMAD3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
别名:MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S204 of human SMAD3 protein. The exact sequence is proprietary.
应用稀释比例：WB: 1-500-1-1000, IF/ICC: 1-100-1-500, IP: 1-10-1-100
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:17127, 25631, 4088
UniProt ID:P84025, P84022, Q8BUN5
Note:For research use only.
别名:MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S204 of human SMAD3 protein. The exact sequence is proprietary.
应用稀释比例：WB: 1-500-1-1000, IF/ICC: 1-100-1-500, IP: 1-10-1-100
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:17127, 25631, 4088
UniProt ID:P84025, P84022, Q8BUN5
Note:For research use only.
Western blot analysis of SMAD3 (Phospho-S204) expression in HCT116 (A), A549 (B) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 52 kD)
Immunohistochemical analysis of SMAD3 (Phospho-S204) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SMAD3 (Phospho-S204) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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SMAD3 (Phospho-S204) Antibody抗体，orb214215，biorbyt
￥1820
