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- 详细信息
- 技术资料
- 抗体名:
LCK (Phospho-Y393) Antibody抗体
- 抗体英文名:
LCK (Phospho-Y393) Antibody
- 应用范围:
WB
- 宿主:
Rabbit
- 适应物种:
Bovine, Gallus, Human, Mouse, Porcine, Rat, Sheep
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb214180
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原:
KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y393 of human LCK protein. The exact sequence is proprietary.
- 规格:
30 ul
产品描述:The LCK (Phospho-Y393) Antibody is suitable for WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y393 of human LCK protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
别名:Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y393 of human LCK protein. The exact sequence is proprietary.
应用稀释比例:WB: 1-500-1-1000, IF/ICC: 1-100-1-500
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:313050, 16818, 3932
UniProt ID:P06240, Q01621, P06239
Note:For research use only.



别名:Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y393 of human LCK protein. The exact sequence is proprietary.
应用稀释比例:WB: 1-500-1-1000, IF/ICC: 1-100-1-500
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:313050, 16818, 3932
UniProt ID:P06240, Q01621, P06239
Note:For research use only.

Western blot analysis of LCK (Phospho-Y393) expression in Myla2059 (A), HuT78 (B) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 56 kD)

Immunofluorescent analysis of LCK (Phospho-Y393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Direct ELISA antibody dose-response curve using Anti-LCK (Phospho-Y393) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
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LCK (Phospho-Y393) Antibody抗体,orb214180,biorbyt
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