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Gamma-catenin Antibody抗体,orb21

4145,biorbyt
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  • ¥1560
  • biorbyt已认证
  • orb214145
  • 英国
  • 2025年12月05日
  • IF, IHC, WB
  • Rabbit
  • Human, Mouse, Rabbit, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Gamma-catenin Antibody抗体

    • 抗体英文名

      Gamma-catenin Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rabbit, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb214145

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Gamma-catenin. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The Gamma-catenin Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Gamma-catenin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:CTNNG; DP3; Junction plakoglobin; Catenin gamma; Desmoplakin III; Desmoplakin-3

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Gamma-catenin. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:16480, 3728, 81679

    UniProt ID:Q02257, P14923, Q6P0K8

    Note:For research use only.

    产品细节图片1
    Western blot analysis of Gamma-catenin expression in HEK293T (A), Hela (B), H1688 (C), mouse liver (D), rat liver (E) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 83 kD)

    产品细节图片2
    Immunohistochemical analysis of Gamma-catenin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of Gamma-catenin staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    图标文献和实验
    相关实验
    • 【求助】怎么才能获取beta catenin的基因?

      liqiuling 怎么才能获取beta catenin的基因? chuckdouble pubmed里面有Nucleotide.到那里找. liqiuling 不太会看,而且合成的话,很贵,想知道如果想获得别人的惠赠,该怎样做?哪位大侠支个招?不胜感激! lmnsmu 在NCBI的核酸库中找到其序列,然后设计引物,通过RT-PCR扩增获得该基因

    • 【求助】beta-catenin 的表达!

      是你实验过程中的问题。每次实验注意做对照。另外,你的b-catenin的表达是过量表达还是内源的呢?如果内源,适当增加点上样量看看。抗体也需要注意。总之,一切小心,呵呵。 familar woxingwosu wrote: 不一定要提核蛋白才能出来。b-catenin在核内核外都有分布。应该是你实验过程中的问题。每次实验注意做对照。另外,你的b-catenin的表达是过量表达还是内源的呢?如果内源,适当增加点上样量看看。抗体

    • Gamma Irradiation of PMEF

      Irradiating Cells - Once cells are confluent tighten the lid of the flasks and take up to the gamma source in the animal house on the third floor (PMCI), if you have a number of flasks you can trypsinise all the flasks and place

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