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COPS3 Antibody [KO/KD Validate

d]抗体,orb214801,biorbyt
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  • ¥1560
  • biorbyt已认证
  • orb214801
  • 英国
  • 2025年12月05日
  • IF, IHC, WB
  • Rabbit
  • Bovine, Canine, Human, Monkey, Mouse, Porcine, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      COPS3 Antibody [KO/KD Validated]抗体

    • 抗体英文名

      COPS3 Antibody [KO/KD Validated]

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Bovine, Canine, Human, Monkey, Mouse, Porcine, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb214801

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human COPS3. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The COPS3 Antibody [KO/KD Validated] is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human COPS3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:CSN3; COP9 signalosome complex subunit 3; SGN3; Signalosome subunit 3; JAB1-containing signalosome subunit 3

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human COPS3. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:8533, 26572, 287367

    UniProt ID:O88543, Q9UNS2, Q68FW9

    Note:For research use only.

    产品细节图片1
    Western blot analysis of COPS3 expression in H1688 (A), A549 (B), mouse kidney (C), rat kidney (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 48 kD)

    产品细节图片2
    Immunohistochemical analysis of COPS3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of COPS3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    图标文献和实验
    相关实验
    • 一篇搞定 IP、co-IP 实验

      糖,建议进行 Pre-clear;如果使用磁珠,此步可忽略 抗 X 抗体的轻链(25 kD)和重链(50 kD) 更侧重于如何在实验上进行优化(见下文) *注意:总蛋白上样量过多也会造成 非特异性结合,建议上样量为 500 ug-1 mg。上样之前一定要先用 BCA 定个量!定个量!定个量! pre-clear:上样前先用裂解液过一遍柱子,减少基质本身对蛋白的吸附 Output:在某些 co-IP 实验中,实验人员会把IP后的上清分别进行诱饵蛋白 X 和靶蛋白 Y 的 WB 检测

    • BAND-SHIFT

      band-shifts; . Band-shift assay for the determination of Kd Determination of the concentration of radiolabeled antibody In parallel tubes, incubate a ‰ 1 µM radiolabeled antibody solution in PBS with increasing concentrations

    • Immunocytology using the HA epitope

      上一篇:Antibody cleanup--清除非特异抗体   下一篇:Large-scale immunofluorescence

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