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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
COPS3 Antibody [KO/KD Validated]抗体
- 抗体英文名:
COPS3 Antibody [KO/KD Validated]
- 应用范围:
IF, IHC, WB
- 宿主:
Rabbit
- 适应物种:
Bovine, Canine, Human, Monkey, Mouse, Porcine, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb214801
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human COPS3. The exact sequence is proprietary.
- 规格:
30 ul
别名:CSN3; COP9 signalosome complex subunit 3; SGN3; Signalosome subunit 3; JAB1-containing signalosome subunit 3
免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human COPS3. The exact sequence is proprietary.
应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:8533, 26572, 287367
UniProt ID:O88543, Q9UNS2, Q68FW9
Note:For research use only.



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文献和实验糖,建议进行 Pre-clear;如果使用磁珠,此步可忽略 抗 X 抗体的轻链(25 kD)和重链(50 kD) 更侧重于如何在实验上进行优化(见下文) *注意:总蛋白上样量过多也会造成 非特异性结合,建议上样量为 500 ug-1 mg。上样之前一定要先用 BCA 定个量!定个量!定个量! pre-clear:上样前先用裂解液过一遍柱子,减少基质本身对蛋白的吸附 Output:在某些 co-IP 实验中,实验人员会把IP后的上清分别进行诱饵蛋白 X 和靶蛋白 Y 的 WB 检测
band-shifts; . Band-shift assay for the determination of Kd Determination of the concentration of radiolabeled antibody In parallel tubes, incubate a ‰ 1 µM radiolabeled antibody solution in PBS with increasing concentrations
Immunocytology using the HA epitope
上一篇:Antibody cleanup--清除非特异抗体 下一篇:Large-scale immunofluorescence
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