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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
mGLUR4 Antibody抗体
- 抗体英文名:
mGLUR4 Antibody
- 应用范围:
IF, IHC, WB
- 宿主:
Rabbit
- 适应物种:
Gallus, Human, Monkey, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb214012
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mGLUR4. The exact sequence is proprietary.
- 规格:
30 ul
别名:GPRC1D; MGLUR4; Metabotropic glutamate receptor 4; mGluR4
免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mGLUR4. The exact sequence is proprietary.
应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:268934, 24417, 2914
UniProt ID:Q14833, P31423, Q68EF4
Note:For research use only.



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文献和实验按以下步骤进行免疫染色: 1. 含1%正常血清或1%BSA的PBS预孵育20~30分钟; 2. 两种特异性第一抗体(免疫自不同种属动物)的混合物4℃下孵育24~48小时;PBS漂洗3次,每次10分钟; 3. 与第一抗体种属相应的纳米金标记的IgGFab,片段和生物素标记的第二抗体的混合物室温孵育2小时或4℃下孵育12小时后PBS漂洗3次,每次10分钟; 4. ABC(1:100,Vector)室温孵育2小时后PBS漂洗3次,每次10分钟。之后PBS漂洗2次,每次10分钟
expressed mGluR subtype is problematic, because pharmacological agents with the required specificity are not currently available. Moreover, other proteins that interact with L -glutamate, such as ionotropic glutamate receptors (1 or glutamate transporters
Isolation of Receptor Clones by Expression Screening in Xenopus Oocytes
, a metabotropic glutamate receptor (mGluR) clone was also identified with the oocyte system (5 ).
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