Histone H3 (Phospho-S10) Rabbit Polyclonal Antibody 抗体,orb214781,Biorbyt产品图

Histone H3 (Phospho-S10) Rabbi

t Polyclonal Antibody 抗体,orb214781,Biorbyt
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  • ¥1872 - 5148
  • Biorbyt已认证
  • orb214781
  • 英国
  • 2026年07月24日
  • IF, IHC, WB
  • Rabbit
  • Bovine, Gallus, Human, Mouse, Porcine, Rat, Zebrafish
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      Histone H3 (Phospho-S10) Rabbit Polyclonal Antibody 抗体

    • 抗体英文名:

      Histone H3 (Phospho-S10) Rabbit Polyclonal Antibody

    • 靶点:

      HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B

    • 应用范围:

      IF, IHC, WB

    • 宿主:

      Rabbit

    • 适应物种:

      Bovine, Gallus, Human, Mouse, Porcine, Rat, Zebrafish

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb214781

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 形态:

      Liquid

    • 免疫原:

      详询

    • 规格:

      30 ul/50 ul/100 ul/200 ul

    规格:30 ul产品价格:¥1872.0
    规格:50 ul产品价格:¥2340.0
    规格:100 ul产品价格:¥3627.0
    规格:200 ul产品价格:¥5148.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: The Histone H3 (Phospho-S10) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S10 of human Histone H3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

    应用稀释比例: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500

    免疫原: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S10 of human Histone H3 protein. The exact sequence is proprietary.

    分子量: 16 kDa

    防腐剂: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    纯化方法: The antibody was purified by immunogen affinity chromatography.

    特异性: Recognizes endogenous levels of Histone H3 protein only when phosphorylated at S10.

    研究领域: Epigenetics & Chromatin

    UniProt ID: P84243, P84245, P68431, Q6LED0, P84228, P68433, P84244, Q71DI3

    Entrez 基因 ID: 8354, 100361558, 8355, 8350, 8968, 8353, 8351, 15077, 8352, 8357, 8356, 319152, 8358, 291159, 15078

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of Histone H3 (Phospho-S10) expression in HEK293T (A), Hela (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

    产品细节图片2
    Immunohistochemical analysis of Histone H3 (Phospho-S10) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of Histone H3 (Phospho-S10) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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