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Annexin A1 Antibody抗体,orb21355

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  • ¥1560
  • biorbyt已认证
  • orb213556
  • 英国
  • 2025年12月05日
  • IF, IHC, WB
  • Rabbit
  • Human, Monkey, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Annexin A1 Antibody抗体

    • 抗体英文名

      Annexin A1 Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Monkey, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb213556

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The Annexin A1 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:ANX1; LPC1; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:301, 16952

    UniProt ID:P10107, P04083

    Note:For research use only.

    产品细节图片1
    Western blot analysis of Annexin A1 expression in HEK293T (A), Hela (B), H446 (C), mouse lung (D), rat lung (E) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 35 kD)

    产品细节图片2
    Immunohistochemical analysis of Annexin A1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of Annexin A1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    图标文献和实验
    相关实验
    • Annexin V-PI双染法

      Annexin V-PI 双染法 在细胞凋亡的早期就有细胞膜表面破损发生。破损时凋亡细胞表面的磷脂腺丝氨酸(PS)可以从细胞内膜翻转至细胞的外膜。该过程发生在DNA片段化之前,因而检测PS的表达,能反映早期凋亡。AnnexinV是相对分子质量为35 000大小的Ca2+依赖的磷脂结合蛋白,对PS有很高的亲和性,并且可以与暴露于细胞外的PS相结合。利用这一原理,可以将AnnexinV标记荧光来识别早期的细胞凋亡。通常利用AnnexinV-FITC和碘化丙啶(P1)来区分凋亡和坏死细胞。PI

    • Tumor Targeting by a Carbohydrate Ligand-Mimicking Peptide

      Annexin A1 (Anxa1) is a highly specific surface marker of tumor vasculature. We used peptide-displaying phage technology to identify a carbohydrate ligand-mimicking 7-mer peptide, IFLLWQR (IF7), which can target Anxa1 in tumor vasculature

    • Use of Polyamine Derivatives as Selective Histone Deacetylase Inhibitors

      suppressor proteins and hyperacetylation of histones H3 and H4. Procedures are also described to examine the ability of PAHAs and PABAs to utilize the polyamine transport system and to induce overexpression of the early apoptotic factor annexin A1.

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