- ￥7722
- Biorbyt
- orb150764
- 英国
- 2026年07月28日
- ICC, IF, IHC, WB
- Mouse
- Human, Mouse, Rat
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- 技术资料
- 抗体名：
Protocadherin Gamma Antibody (RPE) 抗体
- 抗体英文名：
Protocadherin Gamma Antibody (RPE)
- 靶点：
Protocadherin Gamma
- 浓度：
1 mg/ml
- 应用范围：
ICC, IF, IHC, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb150764
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
RPE
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
IgG1
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
描述: Mouse monoclonal to Protocadherin Gamma (RPE). The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes..
克隆号: N159/5 (Formerly sold as S159-5)
别名: PCDH gamma A, PCDH-gamma-A, PCDHGA, Protocadherin gamma A, Protocadherin gamma subfamily A, Gamma Protocadherin A (pan), Pan-Gamma-Protocadherin-A, Pan Gamma Protocadherin A, PCDHGA1, PCDHGA4, PCDHGC4, Protocadherin gamma-A1, Protocadherin gamma-A4, Protocadherin gamma-C4
应用稀释比例: WB (1:1000), ICC/IF (1:100)
应用说明: 1 µg/ml was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
免疫原: Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).
分子量: 100kDa
防腐剂: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
纯化方法: Protein G Purified
特异性: Detects ~100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
研究领域: Metabolism Research
UniProt ID: Q91XZ0
NCBI 参考序列: NP_291062.1
Entrez 基因 ID: 93709
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
描述: Mouse monoclonal to Protocadherin Gamma (RPE). The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes..
克隆号: N159/5 (Formerly sold as S159-5)
别名: PCDH gamma A, PCDH-gamma-A, PCDHGA, Protocadherin gamma A, Protocadherin gamma subfamily A, Gamma Protocadherin A (pan), Pan-Gamma-Protocadherin-A, Pan Gamma Protocadherin A, PCDHGA1, PCDHGA4, PCDHGC4, Protocadherin gamma-A1, Protocadherin gamma-A4, Protocadherin gamma-C4
应用稀释比例: WB (1:1000), ICC/IF (1:100)
应用说明: 1 µg/ml was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
免疫原: Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).
分子量: 100kDa
防腐剂: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
纯化方法: Protein G Purified
特异性: Detects ~100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
研究领域: Metabolism Research
UniProt ID: Q91XZ0
NCBI 参考序列: NP_291062.1
Entrez 基因 ID: 93709
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone N159/5. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Protocadherin Gamma (pan) Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone N159/5. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Protocadherin Gamma (pan) Antibody. (D) Composite.
Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone N159/5. Load: 10 μg. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.
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Protocadherin Gamma Antibody (RPE) 抗体，orb150764，Biorbyt
￥7722
