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- 详细信息
- 技术资料
- 抗体名:
ERAS Antibody (N-term) (F66)抗体
- 抗体英文名:
ERAS Antibody (N-term) (F66)
- 靶点:
ERAS
- 应用范围:
IF, IHC-P, WB
- 宿主:
Rabbit
- 适应物种:
Human
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb1436206
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
参考说明书
- 形态:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- 亚型:
Rabbit IgG
- 免疫原:
Synthetic Peptide
- 规格:
80 ul
产品描述:Rabbit polyclonal antibody to ERAS (F66).
别名:GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, ERAS, HRAS2, HRASP
免疫原:Synthetic Peptide
克隆性:RB13001
分子量:25287
应用稀释比例:IF: 1:100, WB: 1:1000, IHC-P: 1:10~50
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
NCBI:NP_853510.1
UniProt ID:Q7Z444
Note:For research use only.



别名:GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, ERAS, HRAS2, HRASP
免疫原:Synthetic Peptide
克隆性:RB13001
分子量:25287
应用稀释比例:IF: 1:100, WB: 1:1000, IHC-P: 1:10~50
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
NCBI:NP_853510.1
UniProt ID:Q7Z444
Note:For research use only.

Fluorescent confocal image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ERAS (N-term) (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y Cells.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (F66), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of ERAS Antibody (N-term) (F66) in HL60 cell line lysates (35 ug/lane). ERAS (arrow) was detected using the purified Pab.
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ERAS Antibody (N-term) (F66)抗体,orb1436206,biorbyt
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