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- 详细信息
- 技术资料
- 抗体名:
Phospho-H2AX (Ser139) Recombinant Rabbit Monoclonal Antibody抗体
- 抗体英文名:
Phospho-H2AX (Ser139) Recombinant Rabbit Monoclonal Antibody
- 靶点:
H2AX
- 浓度:
1mg/ml
- 应用范围:
IF, IHC-Fr, IHC-P, WB
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb559106
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Recombinant
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
A synthesized peptide derived from human Histone H2A.X around the phosphorylation site of S139 QA-pS-QEY
- 规格:
25 ul
产品描述:Phospho-H2AX (Ser139) Recombinant Rabbit Monoclonal Antibody
别名:Histone H2A.X(S139); phospho-Histone H2A.X(Ser140); H2AX_HUMAN; 2A.X variant histone; H2A.X; H2A/X; H2AFX; Histone H2AX;
免疫原:A synthesized peptide derived from human Histone H2A.X around the phosphorylation site of S139 QA-pS-QEY
预测反应性:Mouse, Rat
克隆性:1C14
分子量:16 kDa
应用稀释比例:WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P16104
Note:For research use only.










别名:Histone H2A.X(S139); phospho-Histone H2A.X(Ser140); H2AX_HUMAN; 2A.X variant histone; H2A.X; H2A/X; H2AFX; Histone H2AX;
免疫原:A synthesized peptide derived from human Histone H2A.X around the phosphorylation site of S139 QA-pS-QEY
预测反应性:Mouse, Rat
克隆性:1C14
分子量:16 kDa
应用稀释比例:WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P16104
Note:For research use only.

293T (H) cells were treated with UV for 5 min, 25 µg total protein per lane of cell lysates probed with Phospho-H2AX (Ser139) monoclonal antibody, unconjugated (orb559106) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Fixative-fixed, paraffin embedded Human Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (orb559106) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Fixative-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (orb559106) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Fixative-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (orb559106) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Fixative-fixed, paraffin embedded Mouse Placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (orb559106) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Fixative-fixed, paraffin embedded Mouse Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (orb559106) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Fixative-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (orb559106) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Fixative-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (orb559106) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Fixative-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (orb559106) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Fixative-fixed, paraffin embedded Rat Placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (orb559106) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
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Phospho-H2AX (Ser139) Recombinant Rabbit Monoclonal Antibody抗体,orb559106,biorbyt
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