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- 详细信息
- 技术资料
- 抗体名:
Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody抗体
- 抗体英文名:
Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody
- 靶点:
KRT19
- 浓度:
1mg/ml
- 应用范围:
IF, IHC-Fr, IHC-P, WB
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb526644
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Recombinant
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
A synthesized peptide derived from human Cytokeratin 19 (370-400aa)
- 规格:
50 ul
产品描述:Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody
别名:40 kDa keratin intermediate filament; CK 19; ck19; CK19; CK-19; KRT19; Cytokeratin19; Cytokeratin-19; k19; k1cs; Keratin 19; Keratin-19; Keratin type I 40 kD; Keratin type i 40kD; Keratin type I cytoskeletal 19; krt19; mgc15366; K1C19_HUMAN; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd
免疫原:A synthesized peptide derived from human Cytokeratin 19 (370-400aa)
预测反应性:Mouse
克隆性:2F3
分子量:44 kDa
应用稀释比例:WB=1:1000-5000, IHC-P=1:500-1000, IHC-F=1:500-1000, IF=1:200-1000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P08727
Note:For research use only.





别名:40 kDa keratin intermediate filament; CK 19; ck19; CK19; CK-19; KRT19; Cytokeratin19; Cytokeratin-19; k19; k1cs; Keratin 19; Keratin-19; Keratin type I 40 kD; Keratin type i 40kD; Keratin type I cytoskeletal 19; krt19; mgc15366; K1C19_HUMAN; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd
免疫原:A synthesized peptide derived from human Cytokeratin 19 (370-400aa)
预测反应性:Mouse
克隆性:2F3
分子量:44 kDa
应用稀释比例:WB=1:1000-5000, IHC-P=1:500-1000, IHC-F=1:500-1000, IF=1:200-1000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P08727
Note:For research use only.

HepG2 cell, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Cytokeratin 19) monoclonal Antibody, Unconjugated (orb526644) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Fixative-fixed, paraffin embedded (human endometrial carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 19) Polyclonal Antibody, Unconjugated (orb526644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Fixative-fixed, paraffin embedded (human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 19) Polyclonal Antibody, Unconjugated (orb526644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Fixative-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 19) Polyclonal Antibody, Unconjugated (orb526644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: MDA-MB-231 (Human) Cell Lysate at 30 ug, Primary: Anti-Cytokeratin 19 (orb526644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 44 kD.
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Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody抗体,orb526644,biorbyt
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