- ￥3120
- biorbyt
- orb500943
- 英国
- 2025年12月04日
- FC, WB
- Rabbit
- Human, Mouse
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- 详细信息
- 技术资料
- 抗体名：
Dnmt3b Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Dnmt3b Rabbit Polyclonal Antibody
- 靶点：
DNMT3B
- 浓度：
1mg/ml
- 应用范围：
FC, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb500943
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human Dnmt3b (251-350/853aa)
- 规格：
50 ul
产品描述：Dnmt3b Rabbit Polyclonal Antibody
别名:Cytosine 5methyltransferase 3B; DNA(cytosine 5) methyltransferase 3 beta; DNA; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; Dnmt3b; Dnmt3 beta; EC 2.1.1.37; ICF; M.HsaIIIB; MGC124407; RP23-89H14.3; DNM3B_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human Dnmt3b (251-350/853aa)
预测反应性:Canine, Equine, Rabbit
分子量:94 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=2μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q9UBC3
Note:For research use only.
别名:Cytosine 5methyltransferase 3B; DNA(cytosine 5) methyltransferase 3 beta; DNA; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; Dnmt3b; Dnmt3 beta; EC 2.1.1.37; ICF; M.HsaIIIB; MGC124407; RP23-89H14.3; DNM3B_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human Dnmt3b (251-350/853aa)
预测反应性:Canine, Equine, Rabbit
分子量:94 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=2μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q9UBC3
Note:For research use only.
Blank control (black line): K562. Primary Antibody (green line): Rabbit Anti-Dnmt3b antibody (orb500943), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control (blue line): HepG2 (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-Dnmt3b antibody (orb500943), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-Dnmt3b (orb500943) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 94 kD, Observed band size: 80/94 kD.
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Dnmt3b Rabbit Polyclonal Antibody抗体，orb500943，biorbyt
￥3120
