- ￥3120
- biorbyt
- orb500902
- 英国
- 2025年12月04日
- FC, WB
- Mouse
- Human
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- 详细信息
- 技术资料
- 抗体名：
CD45 Mouse Monoclonal Antibody抗体
- 抗体英文名：
CD45 Mouse Monoclonal Antibody
- 靶点：
PTPRC
- 浓度：
1mg/ml
- 应用范围：
FC, WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb500902
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 形态：
Liquid
- 亚型：
IgG1
- 免疫原：
Recombinant human CD45
- 规格：
50 ul
产品描述：CD45 Mouse Monoclonal Antibody
别名:B220; CD 45; CD-45; CD45; cd45 antigen; ec3.1.3.48; CD45R; GP180; GP180; GP 180; L CA; LCA; L-CA; Leukocyte common antigen; LY5; Ly-5 glycoprotein; Protein tyrosine phosphatase receptor type C; Protein tyrosine phosphatase receptor type c polypeptide; protein tyrosine phosphatase, receptor type, C; Receptor-type tyrosine-protein phosphatase C; PTPRC; PTPRC_HUMAN; SCID due to PTPRC deficiency; T200; T200 glycoprotein; T200 leukocyte common antigen; Human homolog of severe combined immunodeficiency due to PTPRC deficiency.
免疫原：Recombinant human CD45
克隆性:14A9
分子量:143 kDa
应用稀释比例：WB=1:500-1000, Flow-Cyt=0.5ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein G
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P08575
Note:For research use only.
别名:B220; CD 45; CD-45; CD45; cd45 antigen; ec3.1.3.48; CD45R; GP180; GP180; GP 180; L CA; LCA; L-CA; Leukocyte common antigen; LY5; Ly-5 glycoprotein; Protein tyrosine phosphatase receptor type C; Protein tyrosine phosphatase receptor type c polypeptide; protein tyrosine phosphatase, receptor type, C; Receptor-type tyrosine-protein phosphatase C; PTPRC; PTPRC_HUMAN; SCID due to PTPRC deficiency; T200; T200 glycoprotein; T200 leukocyte common antigen; Human homolog of severe combined immunodeficiency due to PTPRC deficiency.
免疫原：Recombinant human CD45
克隆性:14A9
分子量:143 kDa
应用稀释比例：WB=1:500-1000, Flow-Cyt=0.5ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein G
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P08575
Note:For research use only.
Blank control: U937. Primary Antibody (green line): Mouse Anti-CD45 antibody (orb500902), dilution: 1:50, Secondary Antibody: Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD45) Monoclonal Antibody, Unconjugated (orb500902) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD45) Monoclonal Antibody, Unconjugated (orb500902) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD45) Monoclonal Antibody, Unconjugated (orb500902) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD45) Monoclonal Antibody, Unconjugated (orb500902) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Sample: Lane 1: Molt-4 (Human) Cell Lysate at 30 ug, Lane 2: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-CD45 (orb500902) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 220 kD, Observed band size: 220 kD.
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CD45 Mouse Monoclonal Antibody抗体，orb500902，biorbyt
￥3120
