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- 详细信息
- 技术资料
- 抗体名:
CD74 Antibody (Biotin)抗体
- 抗体英文名:
CD74 Antibody (Biotin)
- 靶点:
CD74
- 浓度:
1 mg/ml
- 应用范围:
ICC, IF, WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb396381
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Biotin
- 克隆性:
Monoclonal
- 亚型:
IgG1
- 免疫原:
Synthetic peptide corresponding to the N-terminal (1-100 aa) of human CD74 conjugated to KLH. Overlaps 100% with all 3 isoforms
- 规格:
100 ug
产品描述:Mouse monoclonal antibody against CD74 conjugated to Biotin.
别名:CD74, DHLAG, HLA DR gamma, HLADG, p33, p35, Protein 41, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), CD74 antigen, CD74 molecule, CD74 molecule, major histocompatibility complex, class II invariant chain, CLIP, Gamma chain of class II antigens, HG2A_HUMAN, HLA class II histocompatibility antigen gamma chain, HLA DR antigens associated invariant chain, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, HLADR antigens associated invariant chain, Ia antigen associated invariant chain, Ia antigen-associated invariant chain, Ia associated invariant chain, Ia gamma, Ii, Invariant polypeptide of major histocompatibility complex class II antigen associated, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA DR gamma chain, MHC HLA-DR gamma chain
免疫原:Synthetic peptide corresponding to the N-terminal (1-100 aa) of human CD74 conjugated to KLH. Overlaps 100% with all 3 isoforms
克隆性:6D9
分子量:33 kDa
应用稀释比例:WB (1:1000); ICC/IF (1:100)
应用注释:A 1:1000 dilution of SMC-269 was sufficient for detection of CD74 in 15 µg of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
防腐剂:136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
纯化:Protein G Purified
保存说明:Conjugated antibodies should be stored according to the product label
NCBI:NP_001020329.1
Entrez:972
UniProt ID:P04233
Note:For research use only.


别名:CD74, DHLAG, HLA DR gamma, HLADG, p33, p35, Protein 41, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), CD74 antigen, CD74 molecule, CD74 molecule, major histocompatibility complex, class II invariant chain, CLIP, Gamma chain of class II antigens, HG2A_HUMAN, HLA class II histocompatibility antigen gamma chain, HLA DR antigens associated invariant chain, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, HLADR antigens associated invariant chain, Ia antigen associated invariant chain, Ia antigen-associated invariant chain, Ia associated invariant chain, Ia gamma, Ii, Invariant polypeptide of major histocompatibility complex class II antigen associated, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA DR gamma chain, MHC HLA-DR gamma chain
免疫原:Synthetic peptide corresponding to the N-terminal (1-100 aa) of human CD74 conjugated to KLH. Overlaps 100% with all 3 isoforms
克隆性:6D9
分子量:33 kDa
应用稀释比例:WB (1:1000); ICC/IF (1:100)
应用注释:A 1:1000 dilution of SMC-269 was sufficient for detection of CD74 in 15 µg of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
防腐剂:136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
纯化:Protein G Purified
保存说明:Conjugated antibodies should be stored according to the product label
NCBI:NP_001020329.1
Entrez:972
UniProt ID:P04233
Note:For research use only.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CD74 Antibody. (D) Composite.

Western Blot analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9. Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.
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CD74 Antibody (Biotin)抗体,orb396381,biorbyt
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