- ￥7410
- biorbyt
- orb181810
- 英国
- 2025年12月04日
- FACS, FC, ICC, IF, IHC, IP, WB
- Mouse
- Bovine, Canine, Guinea pig, Hamster, Human, Mouse, Rat, Sheep
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
HSP27 Antibody (HRP)抗体
- 抗体英文名：
HSP27 Antibody (HRP)
- 靶点：
HSP27
- 浓度：
1 mg/ml
- 应用范围：
FACS, FC, ICC, IF, IHC, IP, WB
- 宿主：
Mouse
- 适应物种：
Bovine, Canine, Guinea pig, Hamster, Human, Mouse, Rat, Sheep
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb181810
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
HRP
- 克隆性：
Monoclonal
- 亚型：
IgG1 Kappa
- 免疫原：
HSP27 peptide
- 规格：
100 ug
别名:HSPB1, HSP27, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, MKBP, DMPK-binding protein
免疫原：HSP27 peptide
克隆性:8A7
分子量:27kDa
应用稀释比例：WB (1:5000), ICC/IF (1:200), IHC (1:100)
应用注释：A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
防腐剂:73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
纯化:Protein G Purified
保存说明:Conjugated antibodies should be stored according to the product label
NCBI:NP_001532.1
Entrez:3315
UniProt ID:P04792
Note:For research use only.
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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