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GABA A Receptor Antibody (RPE)

抗体，orb182048，biorbyt
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  • ￥8580
  • biorbyt已认证
  • orb182048
  • 英国
  • 2025年12月04日
  • AM, ICC, IF, IHC, WB
  • Mouse
  • Human, Mouse, Rat
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      GABA A Receptor Antibody (RPE)抗体

    • 抗体英文名：

      GABA A Receptor Antibody (RPE)

    • 靶点：

      GABA A Receptor

    • 浓度：

      1 mg/ml

    • 应用范围：

      AM, ICC, IF, IHC, WB

    • 宿主：

      Mouse

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb182048

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      RPE

    • 克隆性：

      Monoclonal

    • 亚型：

      IgG1

    • 免疫原：

      Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta 3

    • 规格：

      100 ug

    产品描述：Mouse monoclonal antibody against GABA-A Receptor Beta3, conjugated to RPE.

    别名:ECA5, GABA alpha receptor beta-2 subunit, GABA(A) receptor subunit beta-3, GABAA receptor beta 3 subunit, GABAA receptor subunit beta 3, GABR B3, GABRB3, Gamma aminobutyric acid (GABA) A receptor beta 3, Gamma aminobutyric acid receptor subunit beta 3, Gamma-aminobutyric acid receptor subunit beta-3, GBRB3_HUMAN, MGC9051

    免疫原：Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta 3

    克隆性:N87/25 (Formerly sold as S87-25)

    分子量:55kDa

    应用稀释比例：WB (1:1000), IHC (1:1000), ICC/IF (1:100)

    应用注释：1 µg/ml of SMC-339 was sufficient for detection of Beta 3 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:95.46mM Phosphate, 2.48mM MES and 2mM EDTA

    纯化:Protein G Purified

    保存说明:Conjugated antibodies should be stored according to the product label

    NCBI:NP_032097.1

    Entrez:14402

    UniProt ID:P63080

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody, Clone N87/25. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Beta 3 Antibody (D) Composite.

    产品细节图片2
    Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25. Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.

    产品细节图片3
    Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

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    • （共享）免疫单词缩写及翻译

      缩写 全称 中文 AA autoantibody 自身抗体 AAF aminoacetylfluorescence 氨乙酰基荧光 Ab antibody 抗体 ABC avidin-biotin peroxidase complex 抗生物素（亲合素）-生物素-过氧化物酶复合物 ACh acetycholine 乙酰胆碱 AChR acetycholine receptor 乙酰胆碱受体 AChRAb acetycholine

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