GABA A Receptor Antibody (FITC) 抗体,orb182078,Biorbyt产品图

GABA A Receptor Antibody (FITC

) 抗体,orb182078,Biorbyt
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  • ¥7605
  • Biorbyt已认证
  • orb182078
  • 英国
  • 2026年07月24日
  • AM, ICC, IF, IHC, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      GABA A Receptor Antibody (FITC) 抗体

    • 抗体英文名

      GABA A Receptor Antibody (FITC)

    • 靶点

      GABA A Receptor

    • 浓度

      1 mg/ml

    • 应用范围

      AM, ICC, IF, IHC, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb182078

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      FITC

    • 克隆性

      Monoclonal

    • 保存条件

      详询

    • 亚型

      IgG2A

    • 免疫原

      详询

    • 规格

      100 ug

    类别: Antibodies

    描述: Mouse monoclonal antibody against GABA-A Receptor Alpha-1, conjugated to FITC.

    克隆号: N95/35 (Formerly sold as S95-35)

    别名: ECA4, EJM, EJM5, GABA receptor alpha 1 polypeptide, GABA(A) receptor, GABA(A) receptor subunit alpha 1, GABA(A) receptor subunit alpha-1, GABA(A) receptor, alpha 1, GABRA 1, GABRA1, Gamma aminobutyric acid (GABA) A receptor alpha 1, Gamma aminobutyric acid A receptor alpha 1, Gamma aminobutyric acid receptor subunit alpha 1, Gamma-aminobutyric acid receptor subunit alpha-1, GBRA1_HUMAN

    应用稀释比例: WB (1:1000), IHC (1:1000), ICC/IF (1:100)

    应用说明: 1 µg/ml was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    免疫原: Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1

    分子量: 55kDa

    防腐剂: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

    纯化方法: Protein G Purified

    特异性: Detects ~55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or –Alpha3.

    研究领域: Neuroscience

    UniProt ID: P14867

    NCBI 参考序列: NP_034380.1

    Entrez 基因 ID: 14394

    保存条件: Conjugated antibodies should be stored according to the product label

    备注: For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha-1 Monoclonal Antibody, Clone N95/35. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha-1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha-1 Antibody (D) Composite.

    产品细节图片2
    Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N95/35. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.

    产品细节图片3
    Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N95/35. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

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    图标文献和实验
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      localization of tagged SAX-3 in 293T cell Molecular Probes 2       Alexa Fluor 647 immunocytochemistry   localization of BCG phagosomes   3       Alexa Fluor 647 immunohistochemistry   detect surface DFz2   3       FITC immunohistochemistry   determine

    • Flow Cytometry Assay to Measure Internalization of GPCRs

      , you must rinse the pellet with media to get rid of any residual chelating agents.8) Label receptors with fluorescent antibody -resuspend pellet in 500 µL of media containing 10 mg/mL FITC-M1 (M1 which has been covalently bound to the FITC fluorophore (Molecular

    • Flow Cytometry Assay to Measure Internalization of GPCR's

      the pellet with media to get rid of any residual chelating agents. 8) Label receptors with fluorescent antibody -resuspend pellet in 500 µL of media containing 10 mg/mL FITC-M1 (M1 which has been covalently bound to the FITC fluorophore ( Molecular

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