- ￥8190
- biorbyt
- orb181867
- 英国
- 2025年12月04日
- ICC, IF, WB
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
TRAP1 Antibody (HRP)抗体
- 抗体英文名：
TRAP1 Antibody (HRP)
- 靶点：
TRAP1
- 浓度：
1 mg/ml
- 应用范围：
ICC, IF, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb181867
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
HRP
- 克隆性：
Monoclonal
- 亚型：
IgG1 Kappa
- 免疫原：
Purified recombinant Human TRAP1
- 规格：
100 ug
别名:Heat shock protein 75, Heat shock protein 75 kDa, Heat shock protein 75 kDa mitochondrial, HSP 75, HSP 90L, HSP75, HSP90L, mitochondrial, TNF receptor associated protein 1, TNFR associated protein 1, TNFR-associated protein 1, TRAP 1, TRAP-1, Trap1, TRAP1_HUMAN, Tumor necrosis factor receptor associated protein, Tumor necrosis factor type 1 receptor associated protein, Tumor necrosis factor type 1 receptor-associated protein
免疫原：Purified recombinant Human TRAP1
克隆性:3H4-2H6
分子量:75kDa
应用稀释比例：WB (1:1000), ICC/IF (1:100)
应用注释：1 µg/ml of SMC-207 was sufficient for detection of Trap-1/HSP75 in 20 µg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
防腐剂:73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
纯化:Protein G Purified
保存说明:Conjugated antibodies should be stored according to the product label
NCBI:NP_057376.2
Entrez:10131
UniProt ID:Q12931
Note:For research use only.
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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