- ￥3120
- biorbyt
- orb221937
- 英国
- 2025年12月04日
- FC, IF, IHC-Fr, IHC-P
- Rabbit
- Human
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- 详细信息
- 技术资料
- 抗体名：
TBX5 Rabbit Polyclonal Antibody抗体
- 抗体英文名：
TBX5 Rabbit Polyclonal Antibody
- 靶点：
TBX5
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb221937
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human TBX-5 (1-100/518aa)
- 规格：
50 ul
产品描述：TBX5 Rabbit Polyclonal Antibody
别名:Holt Oram syndrome; HOS; T box 5; T box protein 5; T box transcription factor TBX 5; T box transcription factor TBX5; T-box protein 5; T-box transcription factor TBX5; TBX 5; TBX-5; TBX5_HUMAN; Transcription factor T box 5.
免疫原：KLH conjugated synthetic peptide derived from human TBX-5 (1-100/518aa)
预测反应性:Bovine, Equine, Mouse, Rat, Sheep
分子量:50 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q99593
Note:For research use only.
别名:Holt Oram syndrome; HOS; T box 5; T box protein 5; T box transcription factor TBX 5; T box transcription factor TBX5; T-box protein 5; T-box transcription factor TBX5; TBX 5; TBX-5; TBX5_HUMAN; Transcription factor T box 5.
免疫原：KLH conjugated synthetic peptide derived from human TBX-5 (1-100/518aa)
预测反应性:Bovine, Equine, Mouse, Rat, Sheep
分子量:50 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q99593
Note:For research use only.
Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-TBX5 antibody (orb221937), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (Human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TBX-5) Polyclonal Antibody, Unconjugated (orb221937) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
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TBX5 Rabbit Polyclonal Antibody抗体，orb221937，biorbyt
￥3120
