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- 详细信息
- 技术资料
- 抗体名:
Phospho-BRAF (Ser446) Rabbit Polyclonal Antibody抗体
- 抗体英文名:
Phospho-BRAF (Ser446) Rabbit Polyclonal Antibody
- 靶点:
BRAF
- 浓度:
1mg/ml
- 应用范围:
IF, IHC-Fr, IHC-P
- 宿主:
Rabbit
- 适应物种:
Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb4718
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated Synthesised phosphopeptide derived from human BRAF around the phosphorylation site of Ser446 RD(p-S)SD
- 规格:
50 ul
产品描述:Phospho-BRAF (Ser446) Rabbit Polyclonal Antibody
别名:B Raf(phospho S446); p-B Raf(phospho S446); BRAF_HUMAN; Serine/threonine-protein kinase B-raf; EC:2.7.11.1; BRAF1; RAFB1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; B-Raf proto-oncogene, serine/threonine kinase; NS7; B-raf; B-RAF1; BRAF-1; 94 kDa B raf protein;
免疫原:KLH conjugated Synthesised phosphopeptide derived from human BRAF around the phosphorylation site of Ser446 RD(p-S)SD
预测反应性:Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit
分子量:94 kDa
应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P15056
Note:For research use only.



别名:B Raf(phospho S446); p-B Raf(phospho S446); BRAF_HUMAN; Serine/threonine-protein kinase B-raf; EC:2.7.11.1; BRAF1; RAFB1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; B-Raf proto-oncogene, serine/threonine kinase; NS7; B-raf; B-RAF1; BRAF-1; 94 kDa B raf protein;
免疫原:KLH conjugated Synthesised phosphopeptide derived from human BRAF around the phosphorylation site of Ser446 RD(p-S)SD
预测反应性:Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit
分子量:94 kDa
应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P15056
Note:For research use only.

paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-B-Raf (Ser446)) Polyclonal Antibody, Unconjugated (orb4718) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Fixative-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-B-Raf (Ser446)) Polyclonal Antibody, Unconjugated (orb4718) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Tissue/Cell: rat testis tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-B-Raf (Ser446) Polyclonal Antibody, Unconjugated (orb4718) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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Phospho-BRAF (Ser446) Rabbit Polyclonal Antibody抗体,orb4718,biorbyt
¥3120










