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Phospho-Beta Catenin (Thr41 +

Ser45) Rabbit Polyclonal Antibody抗体,orb5772,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb5772
  • 英国
  • 2025年12月04日
  • FC, ICC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Phospho-Beta Catenin (Thr41 + Ser45) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-Beta Catenin (Thr41 + Ser45) Rabbit Polyclonal Antibody

    • 靶点

      CTNNB1

    • 浓度

      1mg/ml

    • 应用范围

      FC, ICC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb5772

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human Beta-Catenin around the phosphorylation site of Thr41/Ser45 AT(p-T)TAP(p-S)LS

    • 规格

      50 ul

    产品描述:Phospho-Beta Catenin (Thr41 + Ser45) Rabbit Polyclonal Antibody

    别名:CTNB1_HUMAN; Catenin beta-1; catenin beta 1; CTNNB1; Catenin beta; Beta-catenin; CTNNB; OK/SW-cl.35; PRO2286; EVR7; MRD19; NEDSDV; armadillo; βcatenin; β catenin; β-catenin;

    免疫原:KLH conjugated Synthesised phosphopeptide derived from human Beta-Catenin around the phosphorylation site of Thr41/Ser45 AT(p-T)TAP(p-S)LS

    预测反应性:Bovine, Equine, Gallus, Mouse, Porcine, Rabbit

    分子量:86 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P35222

    Note:For research use only.

    产品细节图片1
    Blank control (blue): Hela (fixed with 2% Fixative (10 min), then permeabilized with 0.3% tritionx-100 for 5 min at room temperature). Primary Antibody: Rabbit Anti-Phospho-Beta Catenin (Thr41 + Ser45) antibody (orb5772, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC), dilution: 1:200 in 1X PBS containing 0.5% BSA.

    产品细节图片2
    Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-Beta Catenin (Thr41 + Ser45)) polyclonal Antibody, Unconjugated (orb5772) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片3
    Fixative-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Beta Catenin (Thr41 + Ser45)) Polyclonal Antibody, Unconjugated (orb5772) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Catenin beta-1) Polyclonal Antibody, Unconjugated (orb5772) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

    产品细节图片5
    Tissue/Cell: human kidney tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-Beta Catenin (Thr41/Ser45) Polyclonal Antibody, Unconjugated (orb5772) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
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    • 【求助】关于gsk3-beta功能的疑问

      的关系理不出头绪。GSK-3 beta 总蛋白减少,p-GSK-3 beta (ser 9)增加,是不是足以说明有活性的GSK-3 beta 减少更多,这样理解不知道对不对,希望给以指点。 galaxy22 楼主你好,请问你做出结果的tyr216的抗体是哪个公司的。。。我做出来总量是减少的,active B catenin是减少的,那我有必要做ser9 和tyr216的磷酸化形式吗?我想研究wnt和akt。。。 棉花糖的温柔

    • 【求助】抗体名称Ab-33是什么意思?

      around the phosphorylation site of serine 33 (L-D-SP-G-I)。 http://www.genscript.com/product_001/rec_antibody/code/A00584/category/antibody/Rabbit_Anti_beta-Catenin_Ab-33_polyclonal.html bettyzyj http://www.signalwayantibody.com/resurgam/uppdf/21211.pdf

    • v468 chapter 5 糖原合成酶激酶-3 的抑制

      antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San

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